pSEMCatR1: procaryotic-eucaryotic shuttle vector compatible with pUR and λgt11 expression systems
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چکیده
منابع مشابه
Purification and characterization of a Bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells.
The adhesin of Bacteroides loeschei PK1295 that mediates coaggregation with Streptococcus sanguis 34 and hemagglutination of erythrocytes was purified to electrophoretic homogeneity. The lectinlike protein has an estimated native Mr of 450,000 and consists of six subunits of identical molecular weight (Mr 75,000). The purified adhesin appears to be a basic protein with a pI between 7.4 and 8.0....
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This paper reports the N-terminal amino acid of the product of in vitro translation of satellite tobacco necrosis virus ribonucleic acid (STNV-RNA) by both a procaryotic (Escherichia coli) and eucaryotic (wheat embryo) system. In vitro translation of satellite tobacco necrosis virus RNA by the procaryotic (Escherichia coli) system initiates with fMet-tRNAyet. Specifically, deformylation of the ...
متن کاملPrimary structure of T4 DNA polymerase. Evolutionary relatedness to eucaryotic and other procaryotic DNA polymerases.
Bacteriophage T4 gene 43 codes for the viral DNA polymerase. We report here the sequence of gene 43 and about 70 nucleotides of 5'- and 3'-flanking sequences, determined by both DNA and RNA sequencing. We have also purified T4 DNA polymerase from T4 infected Escherichia coli and from E. coli containing a gene 43 overexpression vector. A major portion of the deduced amino acid sequence has been ...
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Cyanocycline A was cytotoxic against Meth A cells in vitro, and also showed marked activity against the same cell line grown as an experimental ascites tumor. This antibiotic inhibited nucleic acid synthesis in Escherichia coli and Meth A cells. The antimicrobial activity of the antibiotic was reversed by addition of exogenous herring sperm DNA. Cytofluorometric analysis of cyanocycline A-treat...
متن کاملNovel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
DNA polymerases catalyze the addition of deoxyribonucleotides onto DNA primers in a template-directed manner. The requirement for template instruction distinguishes these enzymes from other nucleotidyl transferases, such as terminal deoxynucleotidyl transferase, that do not utilize a template. An oligonucleotide substrate was used to characterize a novel, non-templated nucleotide addition react...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.21.9090